FBHE

规格:

货期:

编号:TS212171

品牌:Testobio

产品名称：	FBHE
商品货号：	TS212171
Organism：	Bos taurus, cow
Tissue：	heart; vascular endothelium
Cell Type：	endothelial
Product Format：	frozen
Morphology：	endothelial
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain bovine vral diarrhea virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	fetus
Applications：	The line is positive for bovine viral diarrhea virus (BVD).
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE).
Comments：	The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE). The line is positive for bovine viral diarrhea virus (BVD).
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose (ATCC^® No. 30-2002) and supplemented with 50 μg/mLxa0bovine pituitary extract, 90%; bovine calf serum (ATCC^®No. 30-2030), 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Subculture every 5 to 7 days, before 90% confluency. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:5 Medium Renewal: Twice a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	DJ Gospodarowicz
Deposited As：	Bos taurus
References：	Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976. PubMed: 1069301 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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FBHE

货号: TS212171
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产品名称：	FBHE
商品货号：	TS212171
Organism：	Bos taurus, cow
Tissue：	heart; vascular endothelium
Cell Type：	endothelial
Product Format：	frozen
Morphology：	endothelial
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain bovine vral diarrhea virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	fetus
Applications：	The line is positive for bovine viral diarrhea virus (BVD).
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE).
Comments：	The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE). The line is positive for bovine viral diarrhea virus (BVD).
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose (ATCC^® No. 30-2002) and supplemented with 50 μg/mLxa0bovine pituitary extract, 90%; bovine calf serum (ATCC^®No. 30-2030), 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Subculture every 5 to 7 days, before 90% confluency. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:5 Medium Renewal: Twice a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	DJ Gospodarowicz
Deposited As：	Bos taurus
References：	Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976. PubMed: 1069301 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.