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FAT 7
FAT 7
规格:
货期:
编号:TS212185
品牌:Testobio
产品名称： FAT 7
商品货号： TS212185
Organism： Rattus norvegicus, rat
Tissue： nasal
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： squamous cell carcinoma
Age： adult
Gender： male
Applications：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Storage Conditions： liquid nitrogen vapor phase
Derivation：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
Clinical Data：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
male
Receptor Expression：
epidermal growth factor (EGF)
Genes Expressed：
transforming growth factor alpha (TGF alpha)
Cellular Products：
transforming growth factor alpha (TGF alpha)
Tumorigenic： Yes
Effects：
Yes, in female CD-1 nude mice
Comments：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Complete Growth Medium： Hams F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%
Subculturing： Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：

Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time： 24 hrs
Name of Depositor： E Bermudez
Deposited As： Rattus sp.
References：

Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. PubMed: 8148052

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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FAT 7

  • 货号: TS212185
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： FAT 7
商品货号： TS212185
Organism： Rattus norvegicus, rat
Tissue： nasal
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： squamous cell carcinoma
Age： adult
Gender： male
Applications：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Storage Conditions： liquid nitrogen vapor phase
Derivation：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
Clinical Data：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
male
Receptor Expression：
epidermal growth factor (EGF)
Genes Expressed：
transforming growth factor alpha (TGF alpha)
Cellular Products：
transforming growth factor alpha (TGF alpha)
Tumorigenic： Yes
Effects：
Yes, in female CD-1 nude mice
Comments：
The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an adult male rat.
The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).
Complete Growth Medium： Hams F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%
Subculturing： Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：

Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time： 24 hrs
Name of Depositor： E Bermudez
Deposited As： Rattus sp.
References：

Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. PubMed: 8148052

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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