Detroit 539

规格:

货期:

编号:TS212259

品牌:Testobio

产品名称：	Detroit 539
商品货号：	TS212259
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Down syndrome
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	Detroit 539 was derived by W.E. Simpson, W.D. Peterson, Jr., and C.S. Stulberg in December, 1964, from a female Caucasian child with Downs syndrome.
Clinical Data：	female Caucasian
Comments：	Karyotypic analysis of the cell population shows that a major portion of the cell population (approximately 90%) possesses 47 chromosomes, with 5 chromosomes belonging to the G group. These karyotypic findings have been found to persist essentially unchanged through at least 30 serial subcultures. Note: xa0Cytogenetic information is based on initial seed stock at ATCC.
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, sodium pyruvate (1 mM) and 0.1% lactalbumin hydrolysate, 90%; fetal bovine serum, 10%. Maintain pH at 7.3 to 7.4
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isoenzymes：	G6PD, B
Name of Depositor：	CS Stulberg
Deposited As：	Homo sapiens
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

首页 > 产品中心 > 微生物培养 > 菌株 > null > Detroit 539

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Detroit 539

货号: TS212259
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Detroit 539
商品货号：	TS212259
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Down syndrome
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	Detroit 539 was derived by W.E. Simpson, W.D. Peterson, Jr., and C.S. Stulberg in December, 1964, from a female Caucasian child with Downs syndrome.
Clinical Data：	female Caucasian
Comments：	Karyotypic analysis of the cell population shows that a major portion of the cell population (approximately 90%) possesses 47 chromosomes, with 5 chromosomes belonging to the G group. These karyotypic findings have been found to persist essentially unchanged through at least 30 serial subcultures. Note: xa0Cytogenetic information is based on initial seed stock at ATCC.
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, sodium pyruvate (1 mM) and 0.1% lactalbumin hydrolysate, 90%; fetal bovine serum, 10%. Maintain pH at 7.3 to 7.4
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isoenzymes：	G6PD, B
Name of Depositor：	CS Stulberg
Deposited As：	Homo sapiens
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.