Detroit 548

规格:

货期:

编号:TS212263

品牌:Testobio

产品名称：	Detroit 548
商品货号：	TS212263
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast, cytogenetic abnormality
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	infant
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	partial D group trisomy; 46,XX,C+,D-; C+ results from D - D translocation
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, sodium pyruvate (1 mM) and lactalbumin hydrolysate (0.1%), 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:11 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isoenzymes：	G6PD, B
Name of Depositor：	CS Stulberg
Deposited As：	Homo sapiens
References：	Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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Detroit 548

货号: TS212263
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产品名称：	Detroit 548
商品货号：	TS212263
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast, cytogenetic abnormality
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	infant
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	partial D group trisomy; 46,XX,C+,D-; C+ results from D - D translocation
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, sodium pyruvate (1 mM) and lactalbumin hydrolysate (0.1%), 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:11 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isoenzymes：	G6PD, B
Name of Depositor：	CS Stulberg
Deposited As：	Homo sapiens
References：	Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.