DH82

规格:

货期:

编号:TS212265

品牌:Testobio

产品名称：	DH82
商品货号：	TS212265
Organism：	Canis familiaris, dog
Cell Type：	Macrophage-like
Product Format：	frozen
Morphology：	macrophage
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	malignant histiocytosis
Age：	10 years
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Clinical Data：	dog; golden retriever male 10 years
Comments：	The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles. They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors. The cells do not produce IL-1.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	culture medium 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
Name of Depositor：	The United States of America
U.S. Patent Number：	5,192,679
References：	Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993 Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786

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DH82

货号: TS212265
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产品名称：	DH82
商品货号：	TS212265
Organism：	Canis familiaris, dog
Cell Type：	Macrophage-like
Product Format：	frozen
Morphology：	macrophage
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	malignant histiocytosis
Age：	10 years
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Clinical Data：	dog; golden retriever male 10 years
Comments：	The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles. They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors. The cells do not produce IL-1.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	culture medium 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
Name of Depositor：	The United States of America
U.S. Patent Number：	5,192,679
References：	Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993 Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786