| 产品名称： | DEC-205 NLDC-145 |
|---|---|
| 商品货号： | TS212271 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with mouse lymph node stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen) |
| Comments： | The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells. The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation. The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells. The cells are capable of being adapted to serum free growth in using Nutridoma medium. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105xa0and 1 x 106xa0cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC®xa0Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2a |
| Name of Depositor： | RM Steinman, G Kraal |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Kraal G, et al. Langerhans cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. PubMed: 3950549 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | DEC-205 NLDC-145 |
|---|---|
| 商品货号： | TS212271 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with mouse lymph node stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen) |
| Comments： | The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells. The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation. The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells. The cells are capable of being adapted to serum free growth in using Nutridoma medium. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105xa0and 1 x 106xa0cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC®xa0Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2a |
| Name of Depositor： | RM Steinman, G Kraal |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Kraal G, et al. Langerhans cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. PubMed: 3950549 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
