| 产品名称： | DDT1-MF-2 |
|---|---|
| 商品货号： | TS212276 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | ductus deferens; smooth muscle |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leiomyosarcoma |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | male |
| Receptor Expression： | androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic |
| Comments： | Growth of DDT1-MF-2 cells is stimulated by androgens and inhibited by glucocorticoids. Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene. Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase. This a deposit of DDT1-MF-2 (see ATCC CRL-1701) for patent purposes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Merck & Co., Inc. |
| Deposited As： | Mesocricetus auratus |
| U.S. Patent Number： | |
| References： | Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | DDT1-MF-2 |
|---|---|
| 商品货号： | TS212276 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | ductus deferens; smooth muscle |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leiomyosarcoma |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | male |
| Receptor Expression： | androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic |
| Comments： | Growth of DDT1-MF-2 cells is stimulated by androgens and inhibited by glucocorticoids. Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene. Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase. This a deposit of DDT1-MF-2 (see ATCC CRL-1701) for patent purposes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Merck & Co., Inc. |
| Deposited As： | Mesocricetus auratus |
| U.S. Patent Number： | |
| References： | Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
