| 产品名称： | DA4-4 |
|---|---|
| 商品货号： | TS212294 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human mu heavy chain |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human mu heavy chain |
| Comments： | Animals were immunized with human IgM. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium, 92.5%; DMSO, 7.5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JF Kearney, H Kubagawa |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Maruyama S, et al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. J. Immunol. 135: 192-199, 1985. PubMed: 3923101 Nahm MH, et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. PubMed: 9291318 |
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| 产品名称： | DA4-4 |
|---|---|
| 商品货号： | TS212294 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human mu heavy chain |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human mu heavy chain |
| Comments： | Animals were immunized with human IgM. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium, 92.5%; DMSO, 7.5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JF Kearney, H Kubagawa |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Maruyama S, et al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. J. Immunol. 135: 192-199, 1985. PubMed: 3923101 Nahm MH, et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. PubMed: 9291318 |
