| 产品名称： | DAL K20 |
|---|---|
| 商品货号： | TS212296 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Applications： | The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human normal tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human normal tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis |
| Comments： | The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2xa0 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | SJ Luner, T Ghose |
| References： | Luner SJ, et al. Monoclonal antibodies to kidney and tumor-associated surface antigens of human renal cell carcinoma. Cancer Res. 46: 5816-5820, 1986. PubMed: 3530441 Guha AK, et al. Tumor localization of monoclonal antibodies against human renal carcinoma in a xenograft model. Cancer Lett. 61: 35-43, 1991. PubMed: 1764696 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | DAL K20 |
|---|---|
| 商品货号： | TS212296 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Applications： | The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human normal tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human normal tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis |
| Comments： | The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2xa0 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | SJ Luner, T Ghose |
| References： | Luner SJ, et al. Monoclonal antibodies to kidney and tumor-associated surface antigens of human renal cell carcinoma. Cancer Res. 46: 5816-5820, 1986. PubMed: 3530441 Guha AK, et al. Tumor localization of monoclonal antibodies against human renal carcinoma in a xenograft model. Cancer Lett. 61: 35-43, 1991. PubMed: 1764696 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
