| 产品名称： | D3-2H2-9-21 |
|---|---|
| 商品货号： | TS212307 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with dengue virus type 4 antigens.
Spleen cells were fused with P3X63Ag8 myeloma cells.
|
| Genes Expressed： | immunoglobulin; monoclonal antibody; against dengue virus complex |
| Cellular Products： | immunoglobulin; monoclonal antibody; against dengue virus complex |
| Comments： | The antibody reacts with all members of the dengue virus complex.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106xa0cells/mL. Medium Renewal: Two to three times weekly. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Isotype： | IgG2a |
| Name of Depositor： | MK Gentry |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | D3-2H2-9-21 |
|---|---|
| 商品货号： | TS212307 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with dengue virus type 4 antigens.
Spleen cells were fused with P3X63Ag8 myeloma cells.
|
| Genes Expressed： | immunoglobulin; monoclonal antibody; against dengue virus complex |
| Cellular Products： | immunoglobulin; monoclonal antibody; against dengue virus complex |
| Comments： | The antibody reacts with all members of the dengue virus complex.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106xa0cells/mL. Medium Renewal: Two to three times weekly. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Isotype： | IgG2a |
| Name of Depositor： | MK Gentry |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
