| 产品名称： | CRFK |
|---|---|
| 商品货号： | TS212322 |
| Organism： | Felis catus, cat |
| Tissue： | kidney, cortex |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | 12 weeks |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Virus Susceptibility： | Feline panleukopenia virus
Feline calicivirus Reovirus (Feline) Felid herpesvirus 1 Canine parvovirus Rabies virus Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1 |
| Comments： | The cells are free of bovine viral diarrhea (BVD) virus. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | RA Van Deusen |
| Deposited As： | Felis catus |
| References： | Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570 Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310 Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781 Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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| 产品名称： | CRFK |
|---|---|
| 商品货号： | TS212322 |
| Organism： | Felis catus, cat |
| Tissue： | kidney, cortex |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | 12 weeks |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Virus Susceptibility： | Feline panleukopenia virus
Feline calicivirus Reovirus (Feline) Felid herpesvirus 1 Canine parvovirus Rabies virus Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1 |
| Comments： | The cells are free of bovine viral diarrhea (BVD) virus. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | RA Van Deusen |
| Deposited As： | Felis catus |
| References： | Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570 Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310 Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781 Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
