| 产品名称： | COLO 320HSR COLO 320 HSR |
|---|---|
| 商品货号： | TS212331 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Product Format： | frozen |
| Morphology： | cells are rounded and refractile |
| Culture Properties： | loosely adherent, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, colorectal adenocarcinoma |
| Age： | 55 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM |
| Clinical Data： | 55 years Caucasian female |
| Genes Expressed： | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Cellular Products： | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | The cells are weakly positive for keratins and vimentin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 8,9 TPOX: 8,9 vWA: 15,18 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 |
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| 产品名称： | COLO 320HSR COLO 320 HSR |
|---|---|
| 商品货号： | TS212331 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Product Format： | frozen |
| Morphology： | cells are rounded and refractile |
| Culture Properties： | loosely adherent, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, colorectal adenocarcinoma |
| Age： | 55 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM |
| Clinical Data： | 55 years Caucasian female |
| Genes Expressed： | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Cellular Products： | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | The cells are weakly positive for keratins and vimentin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 8,9 TPOX: 8,9 vWA: 15,18 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 |
