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CL-S1
CL-S1
规格:
货期:
编号:TS212339
品牌:Testobio
产品名称： CL-S1
商品货号： TS212339
Organism： Mus musculus, mouse
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender： female
Applications：
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions： liquid nitrogen vapor phase
Clinical Data：
female
Genes Expressed：
plasminogen activator (low amounts)
Cellular Products：
plasminogen activator (low amounts)
Comments：
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium： Dulbeccos modified Eagles medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation：
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37.0°C
Name of Depositor： LW Anderson
Deposited As： Mus musculus
References：

Anderson LW, et al. New cell line. Epithelial cell line and subline established from premalignant mouse mammary tissue. In Vitro 15: 841-843, 1979. PubMed: 232056

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CL-S1

  • 货号: TS212339
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： CL-S1
商品货号： TS212339
Organism： Mus musculus, mouse
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender： female
Applications：
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions： liquid nitrogen vapor phase
Clinical Data：
female
Genes Expressed：
plasminogen activator (low amounts)
Cellular Products：
plasminogen activator (low amounts)
Comments：
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium： Dulbeccos modified Eagles medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation：
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37.0°C
Name of Depositor： LW Anderson
Deposited As： Mus musculus
References：

Anderson LW, et al. New cell line. Epithelial cell line and subline established from premalignant mouse mammary tissue. In Vitro 15: 841-843, 1979. PubMed: 232056

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