CHO 1-15 [subscript 500]

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货期:

编号:TS212359

品牌:Testobio

产品名称：	CHO 1-15 subscript 500
商品货号：	TS212359
Organism：	Cricetulus griseus, hamster, Chinese
Tissue：	ovary
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	female
Shipping Information：	Distributed: Frozen
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation：	The line was produced by transfecting CHO cells with a plasmid (pETPFR, ATCC 40403) to produce a system in which human tissue plasminogen activator is produced.xa0 xa0
HeLa Markers：	N
Genes Expressed：	human tissue plasminogen activator (tPA, t-PA)
Cellular Products：	human tissue plasminogen activator (tPA, t-PA)
Comments：	Expresses human tissue plasminogen activator (t-PA) in eukaryotic DHFR deficient cell lines grown in selective media (glycine, hypoxanthine, thymidine deficient).xa0(Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988) Contains a EcoRI/BamHI fragment of about 2.2 kb containing t-PA and a 1.7 kb SacII fragment containing wild-type dihydrofolate reductase under SV40 control.xa0(Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988)
Complete Growth Medium：	Hams F12 medium, 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:5 to 1:10 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	Genentech, Inc.
Deposited As：	Cricetulus griseus
U.S. Patent Number：	4,766,075
References：	Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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CHO 1-15 [subscript 500]

货号: TS212359
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产品名称：	CHO 1-15 subscript 500
商品货号：	TS212359
Organism：	Cricetulus griseus, hamster, Chinese
Tissue：	ovary
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	female
Shipping Information：	Distributed: Frozen
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation：	The line was produced by transfecting CHO cells with a plasmid (pETPFR, ATCC 40403) to produce a system in which human tissue plasminogen activator is produced.xa0 xa0
HeLa Markers：	N
Genes Expressed：	human tissue plasminogen activator (tPA, t-PA)
Cellular Products：	human tissue plasminogen activator (tPA, t-PA)
Comments：	Expresses human tissue plasminogen activator (t-PA) in eukaryotic DHFR deficient cell lines grown in selective media (glycine, hypoxanthine, thymidine deficient).xa0(Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988) Contains a EcoRI/BamHI fragment of about 2.2 kb containing t-PA and a 1.7 kb SacII fragment containing wild-type dihydrofolate reductase under SV40 control.xa0(Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988)
Complete Growth Medium：	Hams F12 medium, 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:5 to 1:10 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	Genentech, Inc.
Deposited As：	Cricetulus griseus
U.S. Patent Number：	4,766,075
References：	Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.