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CHP-212
CHP-212
规格:
货期:
编号:TS212367
品牌:Testobio
产品名称: CHP-212
商品货号: TS212367
Organism: Homo sapiens, human
Tissue: brain
Product Format: frozen
Morphology: neuroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: neuroblastoma
Storage Conditions: liquid nitrogen vapor phase
Complete Growth Medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mMxa0EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:10 to 1:50 every 2 toxa03 weeksxa0
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation:
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,13
D16S539: 13
D5S818: 10,12
D7S820: 11
THO1: 6
TPOX: 8,11
vWA: 15,18
Name of Depositor: JL Biedler
Deposited As: Homo sapiens
首页 > 产品中心 > 微生物培养 > 菌株 > null > CHP-212

CHP-212

  • 货号: TS212367
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: CHP-212
商品货号: TS212367
Organism: Homo sapiens, human
Tissue: brain
Product Format: frozen
Morphology: neuroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: neuroblastoma
Storage Conditions: liquid nitrogen vapor phase
Complete Growth Medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mMxa0EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:10 to 1:50 every 2 toxa03 weeksxa0
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation:
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,13
D16S539: 13
D5S818: 10,12
D7S820: 11
THO1: 6
TPOX: 8,11
vWA: 15,18
Name of Depositor: JL Biedler
Deposited As: Homo sapiens
合作单位: