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Ch 1 Es (NBL-8)
Ch 1 Es (NBL-8)
规格:
货期:
编号:TS212371
品牌:Testobio
产品名称： Ch 1 Es (NBL-8)
商品货号： TS212371
Organism： Capra hircus, goat
Tissue： esophagus
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent
Biosafety Level： 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： normal
Age： 2/3 term
Gender： male
Applications：
The cells are positive for bovine viral diarrhea virus (BVDV).
Storage Conditions： liquid nitrogen vapor phase
Clinical Data：
male
Virus Resistance：
poliovirus 3; coxsackievirus A9, B5; adenovirus 5
Comments：
The cells are positive for bovine viral diarrhea virus (BVDV).
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing： Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Everyxa01 toxa02 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation：

Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：
Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO2)
Name of Depositor： AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens
Deposited As： Capra hircus
References：

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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Ch 1 Es (NBL-8)

  • 货号: TS212371
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Ch 1 Es (NBL-8)
商品货号： TS212371
Organism： Capra hircus, goat
Tissue： esophagus
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent
Biosafety Level： 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： normal
Age： 2/3 term
Gender： male
Applications：
The cells are positive for bovine viral diarrhea virus (BVDV).
Storage Conditions： liquid nitrogen vapor phase
Clinical Data：
male
Virus Resistance：
poliovirus 3; coxsackievirus A9, B5; adenovirus 5
Comments：
The cells are positive for bovine viral diarrhea virus (BVDV).
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing： Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Everyxa01 toxa02 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation：

Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：
Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO2)
Name of Depositor： AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens
Deposited As： Capra hircus
References：

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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