CFPAC-1

规格:

货期:

编号:TS212386

品牌:Testobio

产品名称：	CFPAC-1
商品货号：	TS212386
Organism：	Homo sapiens, human
Tissue：	pancreas; derived from metastatic: liver
Cell Type：	Epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	ductal adenocarcinoma; cystic fibrosis
Age：	26 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	hyperdiploid; modal number = 73; range = 65 to 75; several translocations were evident; two to three apparently normal copies of number 7 (to which the cystic fibrosis gene has been mapped) were present in every metaphase examined
Derivation：	This line was derived from a ductal adenocarcinoma (liver metastasis) from a patient with cystic fibrosis.
Clinical Data：	26 years Caucasian male
Antigen Expression：	CA19-9 antigen, 12000 units/mL, epithelial keratins
Genes Expressed：	carcinoembryonic antigen (CEA), 9 ng/mL; pancreatic oncofetal antigen (POA), 28 ng/mL; adenocarcinoma associated antigen (ACAA), 5000 ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins
Cellular Products：	carcinoembryonic antigen (CEA), 9 ng/mL; pancreatic oncofetal antigen (POA), 28 ng/mL; adenocarcinoma associated antigen (ACAA), 5000 ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins
Tumorigenic：	Yes
Effects：	Yes, in nude mice (passage 34)
Comments：	The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR). CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux. The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508. CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,11 D5S818: 10,11 D7S820: 8,10 THO1: 8 TPOX: 8 vWA: 17
Population Doubling Time：	32 hrs
Name of Depositor：	RA Schoumacher
Deposited As：	Homo sapiens
References：	Schoumacher RA, et al. A cystic fibrosis pancreatic adenocarcinoma cell line. Proc. Natl. Acad. Sci. USA 87: 4012-4016, 1990. PubMed: 1692630 McIntosh JC, et al. Pancreatic adenocarcinoma in a patient with cystic fibrosis. Am. J. Med. 85: 592, 1988. PubMed: 3177424

首页 > 产品中心 > 微生物培养 > 菌株 > null > CFPAC-1

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

CFPAC-1

货号: TS212386
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	CFPAC-1
商品货号：	TS212386
Organism：	Homo sapiens, human
Tissue：	pancreas; derived from metastatic: liver
Cell Type：	Epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	ductal adenocarcinoma; cystic fibrosis
Age：	26 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	hyperdiploid; modal number = 73; range = 65 to 75; several translocations were evident; two to three apparently normal copies of number 7 (to which the cystic fibrosis gene has been mapped) were present in every metaphase examined
Derivation：	This line was derived from a ductal adenocarcinoma (liver metastasis) from a patient with cystic fibrosis.
Clinical Data：	26 years Caucasian male
Antigen Expression：	CA19-9 antigen, 12000 units/mL, epithelial keratins
Genes Expressed：	carcinoembryonic antigen (CEA), 9 ng/mL; pancreatic oncofetal antigen (POA), 28 ng/mL; adenocarcinoma associated antigen (ACAA), 5000 ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins
Cellular Products：	carcinoembryonic antigen (CEA), 9 ng/mL; pancreatic oncofetal antigen (POA), 28 ng/mL; adenocarcinoma associated antigen (ACAA), 5000 ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins
Tumorigenic：	Yes
Effects：	Yes, in nude mice (passage 34)
Comments：	The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR). CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux. The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508. CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,11 D5S818: 10,11 D7S820: 8,10 THO1: 8 TPOX: 8 vWA: 17
Population Doubling Time：	32 hrs
Name of Depositor：	RA Schoumacher
Deposited As：	Homo sapiens
References：	Schoumacher RA, et al. A cystic fibrosis pancreatic adenocarcinoma cell line. Proc. Natl. Acad. Sci. USA 87: 4012-4016, 1990. PubMed: 1692630 McIntosh JC, et al. Pancreatic adenocarcinoma in a patient with cystic fibrosis. Am. J. Med. 85: 592, 1988. PubMed: 3177424