CCD-25Sk

规格:

货期:

编号:TS212410

品牌:Testobio

产品名称：	CCD-25Sk
商品货号：	TS212410
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Glioma
Age：	7 years
Gender：	male
Applications：	The line was derived from normal skin of a patient that died from a high grade glioma.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was derived from normal skin of a patient that died from a high grade glioma.
Clinical Data：	The line was derived from normal skin of a patient that died from a high grade glioma. male
Comments：	The line was derived from normal skin of a patient that died from a high grade glioma. This line is capable of at least 8 population doublings from the thawed ampule.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Let the culture sit at room temperature or at 37°C until cells detach (about 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,14 D16S539: 9,11 D5S818: 12 D7S820: 8,11 THO1: 6,7 TPOX: 11,12 vWA: 17,18

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CCD-25Sk

货号: TS212410
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产品名称：	CCD-25Sk
商品货号：	TS212410
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Glioma
Age：	7 years
Gender：	male
Applications：	The line was derived from normal skin of a patient that died from a high grade glioma.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was derived from normal skin of a patient that died from a high grade glioma.
Clinical Data：	The line was derived from normal skin of a patient that died from a high grade glioma. male
Comments：	The line was derived from normal skin of a patient that died from a high grade glioma. This line is capable of at least 8 population doublings from the thawed ampule.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Let the culture sit at room temperature or at 37°C until cells detach (about 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,14 D16S539: 9,11 D5S818: 12 D7S820: 8,11 THO1: 6,7 TPOX: 11,12 vWA: 17,18