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CCD-1137Sk
CCD-1137Sk
规格:
货期:
编号:TS212418
品牌:Testobio
产品名称: CCD-1137Sk
商品货号: TS212418
Organism: Homo sapiens, human
Tissue: skin; foreskin
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: newborn
Gender: male
Applications:
The line was established from skin taken from normal foreskin.
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established from skin taken from normal foreskin.
Clinical Data:
male
Comments:
The line was established from skin taken from normal foreskin.
Cells senesce after approximately 60 population doublings.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: F.Haq, ATCC
Deposited As: human
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CCD-1137Sk

  • 货号: TS212418
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  • 品牌 : TESTOBIO
产品名称: CCD-1137Sk
商品货号: TS212418
Organism: Homo sapiens, human
Tissue: skin; foreskin
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: newborn
Gender: male
Applications:
The line was established from skin taken from normal foreskin.
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established from skin taken from normal foreskin.
Clinical Data:
male
Comments:
The line was established from skin taken from normal foreskin.
Cells senesce after approximately 60 population doublings.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: F.Haq, ATCC
Deposited As: human
合作单位: