CCD-186Sk
CCD-186Sk
规格:
货期:
编号:TS212420
品牌:Testobio
| 产品名称： | CCD-186Sk |
|---|---|
| 商品货号： | TS212420 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cystic fibrosis |
| Age： | 16 years |
| Gender： | male |
| Clinical Data： | The tissue donor suffered from cystic fibrosis. male |
| Genes Expressed： | Homozygous for the Delta 508 mutation in the CFTR gene. |
| Comments： | The tissue donor suffered from cystic fibrosis. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
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周雪
13372109301
| 产品名称： | CCD-186Sk |
|---|---|
| 商品货号： | TS212420 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cystic fibrosis |
| Age： | 16 years |
| Gender： | male |
| Clinical Data： | The tissue donor suffered from cystic fibrosis. male |
| Genes Expressed： | Homozygous for the Delta 508 mutation in the CFTR gene. |
| Comments： | The tissue donor suffered from cystic fibrosis. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
