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CCD-1100Sk
CCD-1100Sk
规格:
货期:
编号:TS212442
品牌:Testobio
产品名称: CCD-1100Sk
商品货号: TS212442
Organism: Homo sapiens, human
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: 9 years
Gender: female
Ethnicity: Caucasian
Applications:
The line was established from normal skin from the leg.
Derivation:
The line was established from normal skin from the leg.
Clinical Data:
female
Caucasian
9 years
Comments:
The line was established from normal skin from the leg.
Cells from the current passage (2) are capable of at least 26 population doublings.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: A Thompson
Passage History:
Cells from the current passage (2) are capable of at least 26 population doublings.
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CCD-1100Sk

  • 货号: TS212442
  • 好评
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  • 品牌 : TESTOBIO
产品名称: CCD-1100Sk
商品货号: TS212442
Organism: Homo sapiens, human
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: 9 years
Gender: female
Ethnicity: Caucasian
Applications:
The line was established from normal skin from the leg.
Derivation:
The line was established from normal skin from the leg.
Clinical Data:
female
Caucasian
9 years
Comments:
The line was established from normal skin from the leg.
Cells from the current passage (2) are capable of at least 26 population doublings.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: A Thompson
Passage History:
Cells from the current passage (2) are capable of at least 26 population doublings.
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