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CCD-1079Sk
CCD-1079Sk
规格:
货期:
编号:TS212451
品牌:Testobio
产品名称: CCD-1079Sk
商品货号: TS212451
Organism: Homo sapiens, human
Tissue: skin
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Normal
Age: newborn
Gender: male
Derivation:
The line was established from skin taken from normal foreskin.
Clinical Data:
male
newborn
Comments:
The cells senesce after approximately 56 population doublings.
Complete Growth Medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%, fetal bovine serum, 10%
Subculturing: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation:
Culture medium, 95%; DMSO, 5%
STR Profile:
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11,12
D16S539: 9,11
D5S818: 11,12
D7S820: 12
THO1: 6,9.3
TPOX: 10,11
vWA: 17,18
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CCD-1079Sk

  • 货号: TS212451
  • 好评
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  • 品牌 : TESTOBIO
产品名称: CCD-1079Sk
商品货号: TS212451
Organism: Homo sapiens, human
Tissue: skin
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Normal
Age: newborn
Gender: male
Derivation:
The line was established from skin taken from normal foreskin.
Clinical Data:
male
newborn
Comments:
The cells senesce after approximately 56 population doublings.
Complete Growth Medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%, fetal bovine serum, 10%
Subculturing: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation:
Culture medium, 95%; DMSO, 5%
STR Profile:
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11,12
D16S539: 9,11
D5S818: 11,12
D7S820: 12
THO1: 6,9.3
TPOX: 10,11
vWA: 17,18
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