CCD-1072Sk

规格:

货期:

编号:TS212458

品牌:Testobio

产品名称：	CCD-1072Sk
商品货号：	TS212458
Organism：	Homo sapiens, human
Tissue：	foreskin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	newborn
Gender：	male
Applications：	The line was established from skin taken from normal foreskin.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established from skin taken from normal foreskin. Cells senesce after at least 35 population doublings.
Clinical Data：	male
Comments：	The line was established from skin taken from normal foreskin. Cells senesce after at least 35 population doublings.
Complete Growth Medium：	Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y CSF1PO: 10, 11 D13S317: 12,14 D16S539: 12,13 D5S818: 10,13 D7S820: 8,11 THO1: 7 TPOX: 6,10 vWA: 15,19
Name of Depositor：	A Thompson, ATCC
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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CCD-1072Sk

货号: TS212458
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产品名称：	CCD-1072Sk
商品货号：	TS212458
Organism：	Homo sapiens, human
Tissue：	foreskin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	newborn
Gender：	male
Applications：	The line was established from skin taken from normal foreskin.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established from skin taken from normal foreskin. Cells senesce after at least 35 population doublings.
Clinical Data：	male
Comments：	The line was established from skin taken from normal foreskin. Cells senesce after at least 35 population doublings.
Complete Growth Medium：	Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y CSF1PO: 10, 11 D13S317: 12,14 D16S539: 12,13 D5S818: 10,13 D7S820: 8,11 THO1: 7 TPOX: 6,10 vWA: 15,19
Name of Depositor：	A Thompson, ATCC
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.