CCD-1070Sk
CCD-1070Sk
规格:
货期:
编号:TS212459
品牌:Testobio
| 产品名称： | CCD-1070Sk |
|---|---|
| 商品货号： | TS212459 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from skin taken from normal foreskin. |
| Clinical Data： | male
newborn |
| Comments： | Cells senesce after approximately 36 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Capacity： | Cells senesce after approximately 36 population doublings. |
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| 产品名称： | CCD-1070Sk |
|---|---|
| 商品货号： | TS212459 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from skin taken from normal foreskin. |
| Clinical Data： | male
newborn |
| Comments： | Cells senesce after approximately 36 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Capacity： | Cells senesce after approximately 36 population doublings. |
