| 产品名称： | CAR |
|---|---|
| 商品货号： | TS212489 |
| Organism： | Carassius auratus, goldfish |
| Tissue： | fin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Virus Resistance： | poliovirus 1; herpes simplex virus |
| Complete Growth Medium： | The base medium for this cell line is Minimum Essential Medium (Gibco 11575-032). To make the complete growth medium, add the following components to the base medium:xa0 Fetal bovine serum (ATCC® 30-2020™)xa0to a final concentration of 10% |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C Atmosphere: 95% Air, 5% CO2 |
| Name of Depositor： | L Moewus-Kobb |
| Deposited As： | Carassius auratus |
| References： | . . Ann. N.Y. Acad. Sci. 126: 344, 1964. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | CAR |
|---|---|
| 商品货号： | TS212489 |
| Organism： | Carassius auratus, goldfish |
| Tissue： | fin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Virus Resistance： | poliovirus 1; herpes simplex virus |
| Complete Growth Medium： | The base medium for this cell line is Minimum Essential Medium (Gibco 11575-032). To make the complete growth medium, add the following components to the base medium:xa0 Fetal bovine serum (ATCC® 30-2020™)xa0to a final concentration of 10% |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C Atmosphere: 95% Air, 5% CO2 |
| Name of Depositor： | L Moewus-Kobb |
| Deposited As： | Carassius auratus |
| References： | . . Ann. N.Y. Acad. Sci. 126: 344, 1964. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
