| 产品名称： | CATH.a |
|---|---|
| 商品货号： | TS212492 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | neuron |
| Product Format： | frozen |
| Culture Properties： | mixed: adherent and suspension |
| Biosafety Level： | 2 Cells contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | The CATH.a cell line was established from cultures of a tumor that arose in the brain of a transgenic mouse. |
| Genes Expressed： | dopamine; norepinephrine; synaptophysin |
| Tumorigenic： | Yes |
| Effects： | Yes, in syngeneic mice |
| Comments： | The transgene contain the SV40 T antigen coding sequence linked to a 783 bp portion of the rat tyrosine hydroxylase gene.
The cells express tyrosine hydroxylase and dopamine beta-hydroxylase, and contain neurofilaments. |
| Complete Growth Medium： | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001). To make the complete medium add the following components to the base medium (88% final concentration):xa0xa0
|
| Subculturing： | Cultures are a mixture of adherent and suspended cells. Collect the floating cells and transfer to new flasks. Dissociate the attached cells using 0.12% trypsin without EDTA. Remove Trypsin by neutralization and gentle centrifugation followed by resuspension in complete growth medium and add to the suspension cell population.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended |
| Cryopreservation： | Freeze medium: 85% RPMI-1640 (ATCC 30-2001) + 10% FBS (ATCC 30-2020) + 5% DMSO (ATCC 4-X)
Storage Temperature: Liquid nitrogen vapor phase
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | Trustees of Tufts University |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Chikaraishi DM. Expression of a target gene in transgenic mammals with 5 flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997 |
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| 产品名称： | CATH.a |
|---|---|
| 商品货号： | TS212492 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | neuron |
| Product Format： | frozen |
| Culture Properties： | mixed: adherent and suspension |
| Biosafety Level： | 2 Cells contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： | |
| Derivation： | The CATH.a cell line was established from cultures of a tumor that arose in the brain of a transgenic mouse. |
| Genes Expressed： | dopamine; norepinephrine; synaptophysin |
| Tumorigenic： | Yes |
| Effects： | Yes, in syngeneic mice |
| Comments： | The transgene contain the SV40 T antigen coding sequence linked to a 783 bp portion of the rat tyrosine hydroxylase gene.
The cells express tyrosine hydroxylase and dopamine beta-hydroxylase, and contain neurofilaments. |
| Complete Growth Medium： | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001). To make the complete medium add the following components to the base medium (88% final concentration):xa0xa0
|
| Subculturing： | Cultures are a mixture of adherent and suspended cells. Collect the floating cells and transfer to new flasks. Dissociate the attached cells using 0.12% trypsin without EDTA. Remove Trypsin by neutralization and gentle centrifugation followed by resuspension in complete growth medium and add to the suspension cell population.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended |
| Cryopreservation： | Freeze medium: 85% RPMI-1640 (ATCC 30-2001) + 10% FBS (ATCC 30-2020) + 5% DMSO (ATCC 4-X)
Storage Temperature: Liquid nitrogen vapor phase
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | Trustees of Tufts University |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Chikaraishi DM. Expression of a target gene in transgenic mammals with 5 flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997 |
