Caki-1

规格:

货期:

编号:TS212505

品牌:Testobio

产品名称：	Caki-1
商品货号：	TS212505
Organism：	Homo sapiens, human
Tissue：	kidney; Derived from metastatic site: skin
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	clear cell carcinoma
Age：	49 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 68; range = 63 to 71. The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
Images：
Derivation：	Caki-1 was derived from a human tumor.
Clinical Data：	49 years Caucasian male
Antigen Expression：	Blood Type O; Rh-; HLA A9, B12, Bw35
Tumorigenic：	Yes
Effects：	Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters
Comments：	Ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8,11 vWA: 15,17
Isoenzymes：	AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	J Fogh
Deposited As：	Homo sapiens
References：	Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

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Caki-1

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产品名称：	Caki-1
商品货号：	TS212505
Organism：	Homo sapiens, human
Tissue：	kidney; Derived from metastatic site: skin
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	clear cell carcinoma
Age：	49 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 68; range = 63 to 71. The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
Images：
Derivation：	Caki-1 was derived from a human tumor.
Clinical Data：	49 years Caucasian male
Antigen Expression：	Blood Type O; Rh-; HLA A9, B12, Bw35
Tumorigenic：	Yes
Effects：	Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters
Comments：	Ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8,11 vWA: 15,17
Isoenzymes：	AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	J Fogh
Deposited As：	Homo sapiens
References：	Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047