| 产品名称： | BNL 1ME A.7R.1 |
|---|---|
| 商品货号： | TS212568 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Cell Type： | epithelial, chemically transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | embryo |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with methylcholanthrene epoxide. |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in BALB/c mice and ATxFL mice |
| Comments： | This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with methylcholanthrene epoxide. The cells will grow in 0.3% agarose. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | P Patek, J Collins, M Cohn |
| Deposited As： | Mus musculus |
| References： | Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 |
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| 产品名称： | BNL 1ME A.7R.1 |
|---|---|
| 商品货号： | TS212568 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Cell Type： | epithelial, chemically transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | embryo |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with methylcholanthrene epoxide. |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in BALB/c mice and ATxFL mice |
| Comments： | This line was derived from BNL CL.2 (ATCC TIB-73) by transformation with methylcholanthrene epoxide. The cells will grow in 0.3% agarose. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | P Patek, J Collins, M Cohn |
| Deposited As： | Mus musculus |
| References： | Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 |
