BNL CL.2

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货期:

编号:TS212569

品牌:Testobio

产品名称：	BNL CL.2
商品货号：	TS212569
Organism：	Mus musculus, mouse
Tissue：	liver
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	embryo
Strain：	BALB/c
Storage Conditions：	liquid nitrogen vapor phase
Tumorigenic：	No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments：	The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: 10% CO₂
Name of Depositor：	P Patek, J Collins, M Cohn
Deposited As：	Mus musculus
References：	Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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BNL CL.2

货号: TS212569
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产品名称：	BNL CL.2
商品货号：	TS212569
Organism：	Mus musculus, mouse
Tissue：	liver
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	embryo
Strain：	BALB/c
Storage Conditions：	liquid nitrogen vapor phase
Tumorigenic：	No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments：	The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: 10% CO₂
Name of Depositor：	P Patek, J Collins, M Cohn
Deposited As：	Mus musculus
References：	Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.