B8-24-3
B8-24-3
规格:
货期:
编号:TS212635
品牌:Testobio
| 产品名称： | B8-24-3 |
|---|---|
| 商品货号： | TS212635 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against H-2 b |
| Cellular Products： | immunoglobulin; monoclonal antibody; against H-2 b |
| Comments： | Animals were immunized with EL4 lymphoma cells mixed with Bordetella pertussis bacteria. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. It does not react with the d, f, j, k, q, s, v, u, w1 and W15 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | G Kohler |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kohler G, et al. Characterization of a monoclonal anti-H-2Kb antibody. Immune Syst. 2: 202-208, 1981. |
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周雪
13372109301
| 产品名称： | B8-24-3 |
|---|---|
| 商品货号： | TS212635 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against H-2 b |
| Cellular Products： | immunoglobulin; monoclonal antibody; against H-2 b |
| Comments： | Animals were immunized with EL4 lymphoma cells mixed with Bordetella pertussis bacteria. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. It does not react with the d, f, j, k, q, s, v, u, w1 and W15 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | G Kohler |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kohler G, et al. Characterization of a monoclonal anti-H-2Kb antibody. Immune Syst. 2: 202-208, 1981. |
