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B16-F10
B16-F10
规格:
货期:
编号:TS212639
品牌:Testobio
产品名称: B16-F10
商品货号: TS212639
Organism: Mus musculus, mouse
Tissue:
skin
Product Format: frozen
Morphology: mixture of spindle-shaped and epithelial-like cells
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: melanoma
Strain: C57BL/6J
Applications:
This line is a suitable transfection host.
Storage Conditions: liquid nitrogen vapor phase
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes are given for a 75 cm2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation:
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions:
    Temperature: 37°C

    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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    B16-F10

    • 货号: TS212639
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: B16-F10
    商品货号: TS212639
    Organism: Mus musculus, mouse
    Tissue:
    skin
    Product Format: frozen
    Morphology: mixture of spindle-shaped and epithelial-like cells
    Culture Properties: adherent
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease: melanoma
    Strain: C57BL/6J
    Applications:
    This line is a suitable transfection host.
    Storage Conditions: liquid nitrogen vapor phase
    Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Subculturing: Volumes are given for a 75 cm2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days
      Cryopreservation:
      Freeze medium: culture medium, 95%; DMSO, 5%
      Storage temperature: liquid nitrogen vapor phase
      Culture Conditions:
      Temperature: 37°C

      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      合作单位: