B13-24

规格:

货期:

编号:TS212643

品牌:Testobio

产品名称：	B13-24
商品货号：	TS212643
Organism：	Cricetulus griseus, hamster, Chinese
Tissue：	ovary
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	2 CELLS CONTAIN SV-40-CMV VIRAL DNA SEQUENCES Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	female
Applications：	The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081). The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation：	The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
Clinical Data：	female
Comments：	This line is Neomycin resistant.xa0 The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).xa0 The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.xa0 The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins. The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Complete Growth Medium：	Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype：	IgG4
Name of Depositor：	ICOS Corporation
Deposited As：	hamster, Chinese
U.S. Patent Number：	5,854,070
References：	Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998 Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158 Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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B13-24

货号: TS212643
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产品名称：	B13-24
商品货号：	TS212643
Organism：	Cricetulus griseus, hamster, Chinese
Tissue：	ovary
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	2 CELLS CONTAIN SV-40-CMV VIRAL DNA SEQUENCES Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	female
Applications：	The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081). The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation：	The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
Clinical Data：	female
Comments：	This line is Neomycin resistant.xa0 The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).xa0 The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.xa0 The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins. The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Complete Growth Medium：	Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype：	IgG4
Name of Depositor：	ICOS Corporation
Deposited As：	hamster, Chinese
U.S. Patent Number：	5,854,070
References：	Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998 Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158 Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.