AtT-20/D16v-F2

规格:

货期:

编号:TS212648

品牌:Testobio

产品名称：	AtT-20/D16v-F2
商品货号：	TS212648
Organism：	Mus musculus, mouse
Tissue：	pituitary, anterior
Product Format：	frozen
Morphology：	small rounded cells
Culture Properties：	loosely adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor
Strain：	LAF1
Applications：	This clone has been used successfully by Moore et al. for several DNA mediated transfection studies relating to endocrine and exocrine secretory pathways. The cells are readily transfected using a standard calcium phosphate protocol.
Storage Conditions：	liquid nitrogen vaor phase
Images：
Genes Expressed：	adrenocorticotropic hormone (ACTH)
Cellular Products：	adrenocorticotropic hormone (ACTH)
Comments：	The F2 subclone of AtT-20 (see ATCC CCL-89) was developed by B. Gumbiner. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Cells grow in patches and pile up. Cultures do not become confluent. Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: Extreme care must be taken to prevent the cells from clumping. Carefully follow the subcultivation protocol .do not over trypsinize. The cells will slough if the pH of the medium becomes too acidic (lower than 7.5). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vaor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	RB Kelly
Deposited As：	Mus musculus
References：	Gumbiner B, Kelly RB. Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59, 1982. PubMed: 6279313 Moore HP, et al. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538, 1983. PubMed: 6317196 Burgess TL, et al. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J. Cell Biol. 101: 639-645, 1985. PubMed: 2991303 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Stefana B, et al. Leukemia inhibitory factor induces differentiation of pituitary corticotroph function: an immuno-neuroendocrine phenotypic switch. Proc. Natl. Acad. Sci. USA 93: 12502-12506, 1996. PubMed: 8901611
Cross References：	Nucleotide (GenBank) : U79773 Mus musculus NNP-1 (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : U79774 Mus musculus NNP-1 var (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : NM_010925 Mus musculus novel nuclear protein 1 (Nnp1), mRNA.

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AtT-20/D16v-F2

货号: TS212648
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产品名称：	AtT-20/D16v-F2
商品货号：	TS212648
Organism：	Mus musculus, mouse
Tissue：	pituitary, anterior
Product Format：	frozen
Morphology：	small rounded cells
Culture Properties：	loosely adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor
Strain：	LAF1
Applications：	This clone has been used successfully by Moore et al. for several DNA mediated transfection studies relating to endocrine and exocrine secretory pathways. The cells are readily transfected using a standard calcium phosphate protocol.
Storage Conditions：	liquid nitrogen vaor phase
Images：
Genes Expressed：	adrenocorticotropic hormone (ACTH)
Cellular Products：	adrenocorticotropic hormone (ACTH)
Comments：	The F2 subclone of AtT-20 (see ATCC CCL-89) was developed by B. Gumbiner. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Cells grow in patches and pile up. Cultures do not become confluent. Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: Extreme care must be taken to prevent the cells from clumping. Carefully follow the subcultivation protocol .do not over trypsinize. The cells will slough if the pH of the medium becomes too acidic (lower than 7.5). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vaor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	RB Kelly
Deposited As：	Mus musculus
References：	Gumbiner B, Kelly RB. Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59, 1982. PubMed: 6279313 Moore HP, et al. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538, 1983. PubMed: 6317196 Burgess TL, et al. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J. Cell Biol. 101: 639-645, 1985. PubMed: 2991303 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Stefana B, et al. Leukemia inhibitory factor induces differentiation of pituitary corticotroph function: an immuno-neuroendocrine phenotypic switch. Proc. Natl. Acad. Sci. USA 93: 12502-12506, 1996. PubMed: 8901611
Cross References：	Nucleotide (GenBank) : U79773 Mus musculus NNP-1 (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : U79774 Mus musculus NNP-1 var (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : NM_010925 Mus musculus novel nuclear protein 1 (Nnp1), mRNA.