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AsPC-1
AsPC-1
规格:
货期:
编号:TS212651
品牌:Testobio
产品名称： AsPC-1
商品货号： TS212651
Organism： Homo sapiens, human
Tissue： pancreas; derived from metastatic site: ascites
Product Format： frozen
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： adenocarcinoma
Age： 62 years
Gender： female
Ethnicity： Caucasian
Applications：
This cell line is a suitable transfection host.
Storage Conditions： liquid nitrogen vapor phase
Derivation：
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Clinical Data：
62 years
Caucasian
female
Genes Expressed：
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Cellular Products：
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Complete Growth Medium： The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation：
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions：
    Temperature: 37°C
    STR Profile：
    Amelogenin: X
    CSF1PO: 10,13
    D13S317: 9,12
    D16S539: 11
    D5S818: 12
    D7S820: 12,13
    THO1: 7,9.3
    TPOX: 8,10
    vWA: 17
    Name of Depositor： MH Tan
    Deposited As： Homo sapiens
    References：

    Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

    Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

    Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

    Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

    Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565

    Cross References：

    Nucleotide (GenBank) : AA640215 01 subtracted 3 EST library Homo sapiens cDNA clone U32, mRNA sequence.

    Nucleotide (GenBank) : AA640216 02 subtracted 3 EST library Homo sapiens cDNA clone U30, mRNA sequence.

    Nucleotide (GenBank) : AA640217 03 subtracted 3 EST library Homo sapiens cDNA clone D32, mRNA sequence.

    Nucleotide (GenBank) : AA640218 04 subtracted 3 EST library Homo sapiens cDNA clone D42, mRNA sequence.

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    AsPC-1

    • 货号: TS212651
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称： AsPC-1
    商品货号： TS212651
    Organism： Homo sapiens, human
    Tissue： pancreas; derived from metastatic site: ascites
    Product Format： frozen
    Culture Properties： adherent
    Biosafety Level： 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
    Disease： adenocarcinoma
    Age： 62 years
    Gender： female
    Ethnicity： Caucasian
    Applications：
    This cell line is a suitable transfection host.
    Storage Conditions： liquid nitrogen vapor phase
    Derivation：
    The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
    Clinical Data：
    62 years
    Caucasian
    female
    Genes Expressed：
    carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
    Cellular Products：
    carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
    Complete Growth Medium： The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
    Subculturing：
    Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
      Medium Renewal: 2 to 3 times per week
      Cryopreservation：
      Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Culture Conditions：
      Temperature: 37°C
      STR Profile：
      Amelogenin: X
      CSF1PO: 10,13
      D13S317: 9,12
      D16S539: 11
      D5S818: 12
      D7S820: 12,13
      THO1: 7,9.3
      TPOX: 8,10
      vWA: 17
      Name of Depositor： MH Tan
      Deposited As： Homo sapiens
      References：

      Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

      Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

      Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

      Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

      Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565

      Cross References：

      Nucleotide (GenBank) : AA640215 01 subtracted 3 EST library Homo sapiens cDNA clone U32, mRNA sequence.

      Nucleotide (GenBank) : AA640216 02 subtracted 3 EST library Homo sapiens cDNA clone U30, mRNA sequence.

      Nucleotide (GenBank) : AA640217 03 subtracted 3 EST library Homo sapiens cDNA clone D32, mRNA sequence.

      Nucleotide (GenBank) : AA640218 04 subtracted 3 EST library Homo sapiens cDNA clone D42, mRNA sequence.

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