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Antibody 2.06
Antibody 2.06
规格:
货期:
编号:TS212669
品牌:Testobio
产品名称: Antibody 2.06
商品货号: TS212669
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with a monomorphic determinant on HLA DR antigens.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against HLA DR
Cellular Products:
immunoglobulin; monoclonal antibody; against HLA DR
Comments:
Animals were immunized with human B lymphocytes.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with a monomorphic determinant on HLA DR antigens.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG1
    Name of Depositor: P Parham
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Charron DJ, McDevitt HO. Analysis of HLA-D region-associated molecules with monoclonal antibody. Proc. Natl. Acad. Sci. USA 76: 6567-6571, 1979. PubMed: 392522

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    Antibody 2.06

    • 货号: TS212669
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: Antibody 2.06
    商品货号: TS212669
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Applications:
    The antibody reacts with a monomorphic determinant on HLA DR antigens.
    Tested and found negative for ectromelia virus (mousepox).
    Derivation:
    Spleen cells were fused with P3X63Ag8 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against HLA DR
    Cellular Products:
    immunoglobulin; monoclonal antibody; against HLA DR
    Comments:
    Animals were immunized with human B lymphocytes.
    Spleen cells were fused with P3X63Ag8 myeloma cells.
    The antibody reacts with a monomorphic determinant on HLA DR antigens.
    Tested and found negative for ectromelia virus (mousepox).
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG1
    Name of Depositor: P Parham
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Charron DJ, McDevitt HO. Analysis of HLA-D region-associated molecules with monoclonal antibody. Proc. Natl. Acad. Sci. USA 76: 6567-6571, 1979. PubMed: 392522

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