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AB1-2
AB1-2
规格:
货期:
编号:TS212713
品牌:Testobio
产品名称: AB1-2
商品货号: TS212713
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue: lymph node
Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain: A/J
Applications:
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
Genes Expressed:
immunoglobulin; monoclonal antibody; against T15 idiotype
Cellular Products:
immunoglobulin; monoclonal antibody; against T15 idiotype
Comments:
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Isotype: mouse IgG1 kappa , IgG1; kappa light chain
Name of Depositor: JF Kearney
Deposited As: mouse (B cell); mouse (myeloma)
References:

Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186

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AB1-2

  • 货号: TS212713
  • 好评
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  • 品牌 : TESTOBIO
产品名称: AB1-2
商品货号: TS212713
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue: lymph node
Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain: A/J
Applications:
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
Genes Expressed:
immunoglobulin; monoclonal antibody; against T15 idiotype
Cellular Products:
immunoglobulin; monoclonal antibody; against T15 idiotype
Comments:
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Isotype: mouse IgG1 kappa , IgG1; kappa light chain
Name of Depositor: JF Kearney
Deposited As: mouse (B cell); mouse (myeloma)
References:

Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186

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