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A6
A6
规格:
货期:
编号:TS212737
品牌:Testobio
产品名称: A6
商品货号: TS212737
Organism: Xenopus laevis, frog, South African clawed
Tissue:
kidney
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: adult
Gender: male
Clinical Data:
male
Virus Resistance:
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies
Complete Growth Medium: NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 26°C
Name of Depositor: KA Rafferty
Deposited As: Xenopus laevis
References:

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803

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A6

  • 货号: TS212737
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  • 品牌 : TESTOBIO
产品名称: A6
商品货号: TS212737
Organism: Xenopus laevis, frog, South African clawed
Tissue:
kidney
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: adult
Gender: male
Clinical Data:
male
Virus Resistance:
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies
Complete Growth Medium: NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 26°C
Name of Depositor: KA Rafferty
Deposited As: Xenopus laevis
References:

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803

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