| 产品名称： | A-204 A204 |
|---|---|
| 商品货号： | TS212757 |
| Organism： | Homo sapiens, human |
| Tissue： | muscle |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | rhabdomyosarcoma |
| Age： | 1 year |
| Gender： | female |
| Karyotype： | diploidy and tetraploidy |
| Clinical Data： | female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms small cell malignant tumor consistent with embryonal rhabdomyosarcoma; also tumorigenic in newborn mice treated with anti thymocyte serum |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,13 D13S317: 11,12 D16S539: 11,12 D5S818: 12 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,9 vWA: 15,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | DJ Giard |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
| Cross References： | Nucleotide (GenBank) : M17560 Simian SRV-1 type D retrovirus (infectious clone) long terminal repeat RNA. Nucleotide (GenBank) : M17561 Simian SRV-1 type D retrovirus (noninfectious clone) long terminal repeat RNA. |
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| 产品名称： | A-204 A204 |
|---|---|
| 商品货号： | TS212757 |
| Organism： | Homo sapiens, human |
| Tissue： | muscle |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | rhabdomyosarcoma |
| Age： | 1 year |
| Gender： | female |
| Karyotype： | diploidy and tetraploidy |
| Clinical Data： | female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms small cell malignant tumor consistent with embryonal rhabdomyosarcoma; also tumorigenic in newborn mice treated with anti thymocyte serum |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,13 D13S317: 11,12 D16S539: 11,12 D5S818: 12 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,9 vWA: 15,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | DJ Giard |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
| Cross References： | Nucleotide (GenBank) : M17560 Simian SRV-1 type D retrovirus (infectious clone) long terminal repeat RNA. Nucleotide (GenBank) : M17561 Simian SRV-1 type D retrovirus (noninfectious clone) long terminal repeat RNA. |
