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9BG5
9BG5
规格:
货期:
编号:TS212762
品牌:Testobio
产品名称: 9BG5
商品货号: TS212762
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Animals were immunized with purified reovirus type 3 virions.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
Cellular Products:
immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
Comments:
Animals were immunized with purified reovirus type 3 virions.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG2a
    Name of Depositor: BN Fields
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Burstin SJ, et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology 117: 146-155, 1982. PubMed: 6175082

    首页 > 产品中心 > 微生物培养 > 菌株 > null > 9BG5

    9BG5

    • 货号: TS212762
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: 9BG5
    商品货号: TS212762
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Applications:
    The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
    Tested and found negative for ectromelia virus (mousepox).
    Derivation:
    Animals were immunized with purified reovirus type 3 virions.
    Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
    Cellular Products:
    immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
    Comments:
    Animals were immunized with purified reovirus type 3 virions.
    Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
    The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
    Tested and found negative for ectromelia virus (mousepox).
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG2a
    Name of Depositor: BN Fields
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Burstin SJ, et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology 117: 146-155, 1982. PubMed: 6175082

    合作单位: