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9D9
9D9
规格:
货期:
编号:TS212767
品牌:Testobio
产品名称: 9D9
商品货号: TS212767
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Antigen Expression:
H-2d
Genes Expressed:
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d
Cellular Products:
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor
Comments:
Mice were immunized with trypsin digested bovine adrenal LDL receptor.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: JL Goldstein
Deposited As: mouse (B cell); mouse (myeloma)
References:

Huettinger M, et al. Use of monoclonal anti-receptor antibodies to probe the expression of the low density lipoprotein receptor in tissues of normal and Watanabe Heritable Hyperlipidemic rabbits. J. Clin. Invest. 74: 1017-1026, 1984. PubMed: 6088578

Kita T, et al. Deficiency of low density lipoprotein receptors in liver and adrenal gland of the WHHL rabbit, an animal model of familial hypercholesterolemia. Proc. Natl. Acad. Sci. USA 78: 2268-2272, 1981. PubMed: 6264472

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9D9

  • 货号: TS212767
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  • 品牌 : TESTOBIO
产品名称: 9D9
商品货号: TS212767
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Antigen Expression:
H-2d
Genes Expressed:
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d
Cellular Products:
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor
Comments:
Mice were immunized with trypsin digested bovine adrenal LDL receptor.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: JL Goldstein
Deposited As: mouse (B cell); mouse (myeloma)
References:

Huettinger M, et al. Use of monoclonal anti-receptor antibodies to probe the expression of the low density lipoprotein receptor in tissues of normal and Watanabe Heritable Hyperlipidemic rabbits. J. Clin. Invest. 74: 1017-1026, 1984. PubMed: 6088578

Kita T, et al. Deficiency of low density lipoprotein receptors in liver and adrenal gland of the WHHL rabbit, an animal model of familial hypercholesterolemia. Proc. Natl. Acad. Sci. USA 78: 2268-2272, 1981. PubMed: 6264472

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