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7G7B6
7G7B6
规格:
货期:
编号:TS212780
品牌:Testobio
产品名称: 7G7B6
商品货号: TS212780
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Influenza
Storage Conditions: liquid nitrogen vapor phase
Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation:
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
Cellular Products:
immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
Comments:
Animals were immunized with influenzavirus stimulated human peripheral blood mononuclear cells.
Spleen cells were fused with Sp2/0 myeloma cells.
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation:
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions:
    Temperature: 37.0°C
    Isotype: IgG2a , IgG2a
    Name of Depositor: The United States of America
    U.S. Patent Number:
    References:

    Biddison W, et al. Soluble interleukin-2 receptor as a disease indicator and a method of assaying the same. US Patent 4,707,443 dated Nov 17 1987

    首页 > 产品中心 > 微生物培养 > 菌株 > null > 7G7B6

    7G7B6

    • 货号: TS212780
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    询价
    • 品牌 : TESTOBIO
    产品名称: 7G7B6
    商品货号: TS212780
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease: Influenza
    Storage Conditions: liquid nitrogen vapor phase
    Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
    Derivation:
    Spleen cells were fused with Sp2/0 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
    Cellular Products:
    immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
    Comments:
    Animals were immunized with influenzavirus stimulated human peripheral blood mononuclear cells.
    Spleen cells were fused with Sp2/0 myeloma cells.
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation:
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions:
    Temperature: 37.0°C
    Isotype: IgG2a , IgG2a
    Name of Depositor: The United States of America
    U.S. Patent Number:
    References:

    Biddison W, et al. Soluble interleukin-2 receptor as a disease indicator and a method of assaying the same. US Patent 4,707,443 dated Nov 17 1987

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