3T6-Swiss albino

规格:

货期:

编号:TS212871

品牌:Testobio

产品名称：	3T6-Swiss albino
商品货号：	TS212871
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	Karyotype unstable within stemline range (78-81). Approximately 21% of the cells contained an extra large chromosome with a terminal centromere and 21% contained minute chromosomes.
Derivation：	The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos.
Genes Expressed：	collagen; hyaluronic acid
Cellular Products：	collagen; hyaluronic acid
Virus Resistance：	poliovirus 2
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell supension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Two to three times a week. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
Cryopreservation：	Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
Name of Depositor：	H Green
Deposited As：	Mus musculus
Year of Origin：	1963
References：	Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Green H, et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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3T6-Swiss albino

货号: TS212871
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产品名称：	3T6-Swiss albino
商品货号：	TS212871
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	Karyotype unstable within stemline range (78-81). Approximately 21% of the cells contained an extra large chromosome with a terminal centromere and 21% contained minute chromosomes.
Derivation：	The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos.
Genes Expressed：	collagen; hyaluronic acid
Cellular Products：	collagen; hyaluronic acid
Virus Resistance：	poliovirus 2
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell supension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Two to three times a week. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
Cryopreservation：	Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
Name of Depositor：	H Green
Deposited As：	Mus musculus
Year of Origin：	1963
References：	Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Green H, et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.