| 产品名称： | 3C7.2 |
|---|---|
| 商品货号： | TS212881 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with G. muris trophozoite surfaces. |
| Derivation： | Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Comments： | Animals were immunized with G. muris trophozoites. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. The antibody reacts with G. muris trophozoite surfaces. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%;
NCTC 109 medium, 10%;
(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);
horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | MF Heyworth |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009 |
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| 产品名称： | 3C7.2 |
|---|---|
| 商品货号： | TS212881 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with G. muris trophozoite surfaces. |
| Derivation： | Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Comments： | Animals were immunized with G. muris trophozoites. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. The antibody reacts with G. muris trophozoite surfaces. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%;
NCTC 109 medium, 10%;
(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);
horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | MF Heyworth |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009 |
