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38.1
38.1
规格:
货期:
编号:TS212891
品牌:Testobio
产品名称: 38.1
商品货号: TS212891
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
Derivation:
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against human CD3
Cellular Products:
immunoglobulin; monoclonal antibody; against human CD3
Comments:
Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
The antibody moderately inhibits alloreactive cytotoxic T lymphocyte activity and is weakly mitogenic for peripheral blood lymphocytes.
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
    Isotype: IgM
    Name of Depositor: PJ Martin
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    . Leucocyte typing: human leucocyte differentiation antig ens detected by monoclonal antibodies. New York: Springer-Verlag; 1984.

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    38.1

    • 货号: TS212891
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: 38.1
    商品货号: TS212891
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Applications:
    The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
    Derivation:
    Spleen cells were fused with NSI/1 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against human CD3
    Cellular Products:
    immunoglobulin; monoclonal antibody; against human CD3
    Comments:
    Animals were immunized with human peripheral blood T lymphocytes.
    Spleen cells were fused with NSI/1 myeloma cells.
    The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
    The antibody moderately inhibits alloreactive cytotoxic T lymphocyte activity and is weakly mitogenic for peripheral blood lymphocytes.
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
    Isotype: IgM
    Name of Depositor: PJ Martin
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    . Leucocyte typing: human leucocyte differentiation antig ens detected by monoclonal antibodies. New York: Springer-Verlag; 1984.

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