3A-sub E [post crisis of 3A(tPA-30-1)]

规格:

货期:

编号:TS212895

品牌:Testobio

产品名称：	3A-sub E post crisis of 3A(tPA-30-1)
商品货号：	TS212895
Organism：	Homo sapiens, human
Tissue：	placenta
Cell Type：	SV40 transformed
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	2 Cells contain polyomavirus DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This line was established from ATCC CRL-1583 cells that had begun to senesce.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	This line was established from ATCC CRL-1583 cells that had begun to senesce.
Genes Expressed：	at 40C the cells produce human chorionic gonadotropin (hCG)
Cellular Products：	at 40C the cells produce human chorionic gonadotropin (hCG)
Comments：	This line was established from ATCC CRL-1583 cells that had begun to senesce. By passage 20 the cells had recovered and appeared capable of unlimited proliferation. The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 33°C. Subcultivation Ratio: 1:2 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 33°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17
Name of Depositor：	MI Cour
Deposited As：	Homo sapiens
Passage History：	By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
References：	Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Reiter J, et al. Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. Placenta 52: 17-20, 2017.

首页 > 产品中心 > 微生物培养 > 菌株 > null > 3A-sub E [post crisis of 3A(tPA-30-1)]

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

3A-sub E [post crisis of 3A(tPA-30-1)]

货号: TS212895
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	3A-sub E post crisis of 3A(tPA-30-1)
商品货号：	TS212895
Organism：	Homo sapiens, human
Tissue：	placenta
Cell Type：	SV40 transformed
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	2 Cells contain polyomavirus DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This line was established from ATCC CRL-1583 cells that had begun to senesce.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	This line was established from ATCC CRL-1583 cells that had begun to senesce.
Genes Expressed：	at 40C the cells produce human chorionic gonadotropin (hCG)
Cellular Products：	at 40C the cells produce human chorionic gonadotropin (hCG)
Comments：	This line was established from ATCC CRL-1583 cells that had begun to senesce. By passage 20 the cells had recovered and appeared capable of unlimited proliferation. The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 33°C. Subcultivation Ratio: 1:2 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 33°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17
Name of Depositor：	MI Cour
Deposited As：	Homo sapiens
Passage History：	By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
References：	Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Reiter J, et al. Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. Placenta 52: 17-20, 2017.