2G8.D6
2G8.D6
规格:
货期:
编号:TS212930
品牌:Testobio
| 产品名称： | 2G8.D6 |
|---|---|
| 商品货号： | TS212930 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium： | Culture Medium: Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | Massachusetts Institute of Technology |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985 |
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| 产品名称： | 2G8.D6 |
|---|---|
| 商品货号： | TS212930 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium： | Culture Medium: Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | Massachusetts Institute of Technology |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985 |
