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2E.6
2E.6
规格:
货期:
编号:TS212931
品牌:Testobio
产品名称: 2E.6
商品货号: TS212931
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against mouse IgG3
Cellular Products:
immunoglobulin; monoclonal antibody; against mouse IgG3
Comments:
Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: Dulbeccos modified Eagles medium with non-essential amino
acids, 90%; horse serum, 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: JM Davie
Deposited As: rat (B cell); mouse (myeloma)
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2E.6

  • 货号: TS212931
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  • 品牌 : TESTOBIO
产品名称: 2E.6
商品货号: TS212931
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against mouse IgG3
Cellular Products:
immunoglobulin; monoclonal antibody; against mouse IgG3
Comments:
Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: Dulbeccos modified Eagles medium with non-essential amino
acids, 90%; horse serum, 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: JM Davie
Deposited As: rat (B cell); mouse (myeloma)
合作单位: