| 产品名称： | 2H-11 |
|---|---|
| 商品货号： | TS212934 |
| Organism： | Mus musculus, mouse |
| Tissue： | axillary lymph node/vascular epithelium |
| Cell Type： | endothelial, SV40 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | Male |
| Strain： | C3H/HeJ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1992 by limiting dilution.
|
| Clinical Data： | male |
| Antigen Expression： | H-2 K; VCAM |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks |
| Comments： | The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay. This clone retains the ability to differentiate on a synthetic basement-like membrane. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen. |
| Complete Growth Medium： | The base medium for this cell line is Dulbeccos Modified Eagles Medium (DMEM; ATCC 30-2002). To make the complete medium add heat-inactivated Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 10%. |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | KA OConnell |
| References： | OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 |
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| 产品名称： | 2H-11 |
|---|---|
| 商品货号： | TS212934 |
| Organism： | Mus musculus, mouse |
| Tissue： | axillary lymph node/vascular epithelium |
| Cell Type： | endothelial, SV40 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | Male |
| Strain： | C3H/HeJ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1992 by limiting dilution.
|
| Clinical Data： | male |
| Antigen Expression： | H-2 K; VCAM |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks |
| Comments： | The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay. This clone retains the ability to differentiate on a synthetic basement-like membrane. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen. |
| Complete Growth Medium： | The base medium for this cell line is Dulbeccos Modified Eagles Medium (DMEM; ATCC 30-2002). To make the complete medium add heat-inactivated Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 10%. |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | KA OConnell |
| References： | OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 |
