25-5-16S
25-5-16S
规格:
货期:
编号:TS212958
品牌:Testobio
| 产品名称： | 25-5-16S |
|---|---|
| 商品货号： | TS212958 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | 0 serologic specificity. The antibody reactivity corresponds to the Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Comments： | Animals were immunized with spleen cells from C3H.SW mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. No cross-reactions are known. The antibody reactivity corresponds to the Ia.20 serologic specificity. Tested and found negative for ectromelia virus (mousepox). The line was recloned in 1988 at the ATCC from the original deposit. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | DH Sachs, K Ozato |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293 |
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周雪
13372109301
| 产品名称： | 25-5-16S |
|---|---|
| 商品货号： | TS212958 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | 0 serologic specificity. The antibody reactivity corresponds to the Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Comments： | Animals were immunized with spleen cells from C3H.SW mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. No cross-reactions are known. The antibody reactivity corresponds to the Ia.20 serologic specificity. Tested and found negative for ectromelia virus (mousepox). The line was recloned in 1988 at the ATCC from the original deposit. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | DH Sachs, K Ozato |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293 |
