22Rv1

规格:

货期:

编号:TS212974

品牌:Testobio

产品名称：	22Rv1
商品货号：	TS212974
Organism：	Homo sapiens, human
Tissue：	prostate
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+125/50,idem,+31
Images：
Derivation：	22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft.
Antigen Expression：	prostate specific antigen (PSA)
Receptor Expression：	androgen receptor
Tumorigenic：	Yes
Effects：	Yes, forms tumors in nude mice
Comments：	The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1). Recently , it has been shown that 22Rv1 prostate carcinoma cells produce high-titer of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,12 D16S539: 12 D5S818: 11,12,13 D7S820: 9,10,11 THO1: 6,9.3 TPOX: 8 vWA: 15,21
Population Doubling Time：	40 hours
Name of Depositor：	JW Jacobberger
Deposited As：	human
References：	Sramkoski RM, et al. A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999. PubMed: 10462204 Knouf EC, et al. Multiple integrated copies and high-level production of the human retrovirus XMRV from 22Rv1 prostate carcinoma cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664

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22Rv1

货号: TS212974
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产品名称：	22Rv1
商品货号：	TS212974
Organism：	Homo sapiens, human
Tissue：	prostate
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+125/50,idem,+31
Images：
Derivation：	22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft.
Antigen Expression：	prostate specific antigen (PSA)
Receptor Expression：	androgen receptor
Tumorigenic：	Yes
Effects：	Yes, forms tumors in nude mice
Comments：	The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1). Recently , it has been shown that 22Rv1 prostate carcinoma cells produce high-titer of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,12 D16S539: 12 D5S818: 11,12,13 D7S820: 9,10,11 THO1: 6,9.3 TPOX: 8 vWA: 15,21
Population Doubling Time：	40 hours
Name of Depositor：	JW Jacobberger
Deposited As：	human
References：	Sramkoski RM, et al. A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999. PubMed: 10462204 Knouf EC, et al. Multiple integrated copies and high-level production of the human retrovirus XMRV from 22Rv1 prostate carcinoma cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664